gen quiz 5 - GENETICS(BIO 325 FALL 2003 IV Quiz(T,Th 9:30...

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Unformatted text preview: GENETICS (BIO 325! FALL 2003 IV Quiz 10/23/03 (T,Th - 9:30 - 11:00 AM) Total points: 16 E. coli strain B is simultaneously infected with two r11 mutants of phage T4. A dilution of the lysate is placed on strain 33. A similar dilution is also placed on strain K. Ten plaques appeared on strain K and [0,000 plaques appeared on strain B. Calculate the percentage recombination between these two mutants. K (4 points) (0 x ; _.___ 0.2% [Om B Fill in the blanks: (4 points) hacomplementahontesa; Monument ritphagesmusedfos demote Mechmoéiflmmifi, (a) nw-fiwmmmnmmn eefiicetlefihemfiflwflam present-in cmfisdififeeemt (smut Meant} gem)». Cb) lithe. infection doesnot result inlysis of the E. coli cells, themutations inthe two [instants are present in. (the) sametsameor. dififerentlgeneés). Explain the differences between complementation and recombination? (4 points) Complementation is an interaction of proteins giving rise to a wild-type phenotype. Recombination involves the interaction of DNA such that the progeny will be diflerent from the parental types. Five point mutations (a through e) in the rIlA region were tested for wild type recombinants with each of the five deletions (1 through 5) shown in the topological map below. The results are listed in the table below. (+ = wild type recombinants, 0 = no recombinants). Determine the order of the point mutations. (4 points) Me I 2 3 4 5 \ I. i .1» a o 0‘ + + + 0 '3 {NI/i a 5w! b + + . + 0' + c 0 3 + + 0 d 07 + D 0 .7 0 e 0 + 0 - 0 7 + a Q 1 C32 63 l9 i 2 a 3 ? ...
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