Bio135_assignment_3

Bio135_assignment_3 - Name Soo-Khee Chia ID 005811923 Biol...

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ID #: 005811923 Biol 135 Assignment #3 Data Handling: (8-47) See question in Molecular Biology of THE CELL (5 th Edition), The Problem Book. A) Fill in Table 8-3 with your expectations. Use a plus sign to indicate growth on plates lacking histidine and a minus sign to indicate no growth. Write ‘blue’ or ‘white’ to indicate the color of colonies grown in the presence of XGAL. PLASMID CONSTRUCTS Growth on Plates Lacking Histidine Color of Plates with XGAL BAIT PREY LexA-Ras - - White LexA-Lamin - - White - VP16 - White - VP16-CYR - White LexA-Ras VP16 - White LexA-Ras VP16-CYR + Blue LexA-Lamin VP16 - White LexA-Lamin VP16-CYR - White B) For any entries in the table that you expect to grow in absence of histidine and form blue colonies with XGAL, sketch the structure of the active transcription factor on the LacZ gene. C) If you want two proteins to be expressed in a single polypeptide chain, what must you be careful to do when you fuse the two genes together? It is important to know that the ‘stop codon’ genes in between the original polypeptide gene
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This note was uploaded on 04/12/2008 for the course BIOL 135 taught by Professor Soto during the Spring '08 term at San Jose State.

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Bio135_assignment_3 - Name Soo-Khee Chia ID 005811923 Biol...

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