6.08 Bacterial Transformation Lab Report.pdf - Bacterial...

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Bacterial Transformation Lab ReportTitle:Bacterial transformation labObjective(s):The length of the time for which the heat shock is given greatly affects the process oftransformation or the uptake of the plasmid by the bacterial cell.Hypothesis:As the length of the time of the heat shock increases there are greater chances of theprocess of transformation or the uptake of the plasmid by the bacterial cell. As the time period of heat shockincreases, the bacterial cell membrane develops pores leading to the entry of the plasmid inside the bacterialcell. Thus the transformation efficiency will increase with an increase in the time period of the heat shocktreatment. However, if the heat shock temperature treatment is increased for longer periods of time then itcan lead to the rupturing of the bacterial cell membrane at very high temperatures for prolonged periods oftime.Materials:Bacterial Transformation Virtual LabSummary of Steps1. Fill all three tubes2. Place tubes into an ice bath3. Inoculate each tube4. Put a colony in each tube5. Swirl the rube6. Record dataGenetic Transformation Reflections Questions1)What is the function of plasmids in bacteria?
2)In previous investigations, you have researched how environmental conditions affect cellularprocesses. From your experience, what conditions do you think will cause disruptions in plasmidtransfer?
The study of Handel et al (2015), suggests that in the absence of the antibiotic, transfer rates were highest,with practically every acceptor cell picking up the plasmid. A common mechanism is hypothesized basedon the mutational pattern of transconjugant cells, which adjusts for fitness costs caused by plasmid transportby lowering other cell activities. Reducing possible fitness costs associated with plasmid maintenance andexpression could help resistance genes persist in the environment even in the absence of antibiotic pressure.

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