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Unformatted text preview: Cleanup SHARPS container only METAL items that could cut or puncture Use of live specimen (bacteria) wipe exposed surfaces of work area w/ 70% ethanol Avoid prolonged contact of lens cleaners and other solvents w/ edges of coverslip dissolve adhesives Many organic solvents are harmful to human tissues never inhale fumes or allow direct skin contact fumehood Bacterial Wastes Microscopes return to lowest objective (4x) lower height of the condenser knobs turned back to original place clean stage/obj of immersion oil (100x) turn off light/power Preparation of Wet Mount drop of culture on slide edge of cover slip a few mm away, gently lower by rotating down onto surface label slide if thick layer of liquid blot edge of slide w/ folded edge of Kimwipe Staining Methylene Blue stains clothes/skin, bacteriological stain DAPI toxic, staining cheek cells for fluorescence microscopy Crystal Violet toxic, stains everything...
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- Fall '07