Chapter 10 - Chapter 10 Recombinant DNA Technology Plant...

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Chapter 10: Recombinant DNA Technology, Plant Biotechnology, and Genomics Multiple-Choice Questions 1. Introduction; p. 180; easy; ans: d Selective breeding programs: a. involve inserting a plasmid into a host cell. b. involve infecting a host plant with a virus. c. allow gene transfers between species incapable of hybridizing. d. depend entirely on genetic recombinations that occur naturally. e. allow the precise insertion of genes into a plant. 2. Recombinant DNA Technology; p. 180; moderate; ans: d A restriction enzyme would most likely be used in recombinant DNA technology to: a. transfer a plasmid into a bacterial cell. b. screen for recombinant plasmids. c. stimulate a transformed cell to divide. d. cut DNA into fragments with sticky ends. e. join DNA fragments together. 3. Recombinant DNA Technology; p. 180; moderate; ans: c Which of the following statements concerning restriction enzymes is FALSE? a. They are a type of endonuclease. b. They recognize DNA sequences typically four to six nucleotides long. c. They recognize single-stranded DNA sequences. d. Some make straight cuts in the DNA. e. They recognize specific palindromic sequences. 4. Recombinant DNA Technology; p. 181; moderate; ans: a A sticky-ended fragment can join with another DNA segment only if that segment: a. has been cut with the same restriction enzyme. b. has been cut with the same ligase. c. is palindromic. d. is part of a plasmid. e. is part of a virus. 5. Recombinant DNA Technology; p. 181; easy; ans: e DNA ligase would most likely be used in recombinant DNA technology to: a. function as a vector. b. screen for recombinant plasmids. c. stimulate a transformed cell to divide. d. cut DNA into fragments with sticky ends. e. join DNA fragments together.
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2 Chapter 10 Recombinant DNA Technology, Plant Biotechnology, and Genomics 6. Recombinant DNA Technology; p. 181; difficult; ans: b By definition, a transformed cell: a. contains a plasmid. b. contains a recombinant DNA molecule. c. contains a virus. d. functions as a vector. e. divides in such a way so as to amplify the DNA fragment. 7. Recombinant DNA Technology; p. 182; moderate; ans: d DNA cloning refers to the process by which: a. plasmids act as vectors. b. restriction enzymes cut up DNA. c. recombinant plasmids are taken up by bacterial cells. d. cells containing a recombinant plasmid divide and grow into a colony. e. colonies are screened to identify the recombinant plasmid. 8. Recombinant DNA Technology; p. 183; moderate; ans: c The amp R gene is used in recombinant DNA technology to: a. produce β-galactosidase. b. clone a particular gene fragment. c. screen for the presence of cells containing recombinant plasmids. d. help the bacterium to survive on a medium containing lactose. e. transform a bacterial cell. 9. Recombinant DNA Technology; p. 183; easy; ans: b Suppose a plasmid containing a gene of interest plus the amp R gene is used to transform E. coli cells.
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