PracticeExamWk5 - Bio180 Fifth Practice Exam Question 1 1....

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Bio180 Fifth Practice Exam Question 1 1. To clean up a Superfund site, you need to find and study bacterial species capable of metabolizing a toxic component of jet fuel called tetradecane. 1) Describe where you would search for such species. 2) Explain how you would isolate them in the lab. 3) In general terms, describe the types of laboratory experiments you would do to identify which nutrient(s) (e.g. nitrogen, potassium, phosphorus) could be added to the site to encourage the growth of tetradecane-metabolizing bacteria. Question 1, Sample answer: 1) Sample soils and/or water from places where tetradecane is abundant—jet fuel spill locations, refining or transport sites, etc. 2) In the lab, put the samples in a growth medium that contains tetradecane and isolate the cells that grow fastest (subsequent work could show whether they are using tetradecane as a carbon source, an electron donor, or an electron acceptor). 3) Expose the cells that grow well on tetradecane to media that contain one extra nutrient (e.g. extra nitrogen or potassium or phosphorus). Compare growth rates in the fertilized treatments versus identical
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This test prep was uploaded on 04/10/2008 for the course BIOL 180 taught by Professor Freeman during the Fall '07 term at University of Washington.

Page1 / 2

PracticeExamWk5 - Bio180 Fifth Practice Exam Question 1 1....

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online