Practice%20Exam%203

Practice%20Exam%203 - Practice Exam 3 1) To quantify and...

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Practice Exam 3 1) To quantify and check the purity of a plasmid DNA sample, you take 1 μ l and add it to 1 ml water in a cuvette. You measure the absorbance in a spectrophotometer and get A = 0.207 at 260 nm and A = 0.123 at 280 nm. At 50 μ g/ml, DNA has an absorbance of 1 at the appropriate wavelength. What is the DNA concentration in μ g/ μ l? Would this plasmid sample be considered free of protein contamination? 2) Using the circle below as a diagram of your pRSET plasmid, make a ‘plasmid map’ of pRSET with your FP gene inserted at the correct restriction sites. On the map, give the size of the ‘vector’ and ‘insert’ segments in base pairs, and make the lengths of these segments as proportional as possible on the map. Assume pRSET is exactly 2900 bp in length. If the FP encoded on the gene is 238 amino acids (all coding region with no stop codons), calculate the size of the insert. Also on your map, show the position of the restriction sites you used to excise the FP insert from your plasmid
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This note was uploaded on 04/13/2008 for the course BIBC 103 taught by Professor Coleman during the Winter '08 term at UCSD.

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Practice%20Exam%203 - Practice Exam 3 1) To quantify and...

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