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Unformatted text preview: 6) What are three things that ensure the proteins loaded onto the gel are completely denatured in SDS-PAGE? 7) On the back of the page, sketch what the gel would look like if you ran a sample containing three proteins, 25 kDa, 44 kDa, and 46 kDa, on SDS-PAGE, and visualized all proteins by staining with coomassie blue. Show the position of each protein band, and label the top of the gel, and positions of the pos. and neg. electrodes. 8) What process produces the voltage across the electric field in electrophoresis? 9) How are monoclonal antibodies produced?...
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This note was uploaded on 04/13/2008 for the course BIBC 103 taught by Professor Coleman during the Winter '08 term at UCSD.
- Winter '08