Practice%20Exam%202

Practice%20Exam%202 - 6) What are three things that ensure...

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BIBC 103 Practice Exam 2 1) What is an antigen? What is an epitope? Can an antigen have more than one epitope? 2) What are the constant region and the variable region of an antibody? 3) What type of post-translational modification occurs on MAP kinase to ‘activate’ it? Where on the MAP kinase protein does this modification occur? 4) In our MAP kinase Western blot: What was the primary antibody? What did it bind to? What was the secondary antibody? What did it bind to? 5) What are the three factors that determine how fast a molecule will migrate in the gel during electrophoresis?
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Unformatted text preview: 6) What are three things that ensure the proteins loaded onto the gel are completely denatured in SDS-PAGE? 7) On the back of the page, sketch what the gel would look like if you ran a sample containing three proteins, 25 kDa, 44 kDa, and 46 kDa, on SDS-PAGE, and visualized all proteins by staining with coomassie blue. Show the position of each protein band, and label the top of the gel, and positions of the pos. and neg. electrodes. 8) What process produces the voltage across the electric field in electrophoresis? 9) How are monoclonal antibodies produced?...
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This note was uploaded on 04/13/2008 for the course BIBC 103 taught by Professor Coleman during the Winter '08 term at UCSD.

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Practice%20Exam%202 - 6) What are three things that ensure...

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