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Exam III 2006 - Name Biology 202 Dec 7 2006 Exam 3 page 2 3...

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Name: Biology 202 ________________________ Dec. 7, 2006 Exam 3 page Value Points 2 10 _____ 3 15 _____ 4 17 _____ 5 13 _____ 6 17 _____ 7 5 _____ 8 8 _____ 9 15 _____ Total 100 _____ The University of Pennsylvania has a Code of Academic Integrity. For this exam, this means that cheating is defined as using or attempting to use unauthorized assistance, material or study aids in this exam, as well as knowingly helping or attempting to help another during the course of this exam. Your signing this exam signifies your pledge to honor and uphold this Code of Academic Integrity during the course of this examination. Signature :_____________________ 1
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Name: BE BRIEF AND CONCISE! BEING BRIEF DECREASES CHANCES OF BEING WRONG WRITE LEGIBLY IN THE SPACE PROVIDED 1. Answer following questions about ubiquitin/proteosome system A. What is the function of ubiquitin? (2 pts) B. What enzymes operate upon ubiquitin and what they do? (3 pts) C. What happens to the molecules conjugated to a chain of several ubiquitins? (2 pts) D. How is a proteosome different from simple proteases? (3 pts) 2. Describe a mechanism by which a protein containing nuclear localization signal becomes accumulated in the nucleus. Mention all important molecules and their functions 2
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Name: (5 pts) . 3. Binding of epinephrine to β -adrenergic receptors in liver cells activates glycogen breakdown to glucose through a cAMP-mediated signaling cascade. Predict and explain the effect on glycogen breakdown of adding (or injecting into cells) the following reagents (2 pts ea) : (A) GMP-PNP (a non-hydrolysable GTP analog) (B) 8-bromo-cAMP (a membrane permeable, less hydrolysable analog of cAMP) (C) Excess regulatory subunit of protein kinase A (D) Excess cAMP phosphodiesterase (E) Caffeine (an inhibitor of cAMP phosphodiesterase).
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