PCR LAB - Jerel O Lee December 5 2013 Polymerase Chain...

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Jerel O. Lee December 5, 2013 Polymerase Chain Reaction (PCR) LAB Dr. Bhaskaran Claflin University Abstract:
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This lab serves a purpose of teaching how to run a successful PCR. It also shows how one can determine if a DNA sample is bacterial by amplifying the 16S gene which amplifies at 1500 Kb. The 16S gene is unique to the domain Eubacteria. If you do not see the 16S gene amplified on the gel then it may be due to contamination from non Eubacteria DNA or from human error. Introduction:
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Polymerase Chain reaction (PCR) was developed to amplify and study genomic DNA (gDNA). Later the method was quickly adapted for studies of mRNA and gene expression using complementary DNA (cDNA) because of the high sensitivity and speed. Only very small amounts of gDNA or cDNA are necessary for the successful detection and isolation of desired DNA fragments or genes. Moreover, the PCR technique has been adopted to sequence the human genome.
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