Exam2Review - How did yall do buttz aweful that I cant even spell right now r LINK to the other Google Doc https\/docs.google.com\/document\/d\/1kZ5VGBpOC03

Exam2Review - How did yall do buttz aweful that I cant even...

This preview shows page 1 - 4 out of 25 pages.

How did y’all do?buttzaweful :( that I can’t even spell right now...r LINK to the other Google Doc:sD9aHy3_l4xjbHX2_kCiAJxUiE16Pa3c/edit^why 2? what is each for? (((MORE INFO=HIGHER GRADE))What time/location is the test again?do yall think we need to know all methods for determining protein concentration? or just absorbance at 280 nm xxxand bradford assay? I’d learn all just to be safe. She said (in class) just the Bradford assay since we didn't learn the othersWhat exactly is the TE buffer? I see it keeps showing
Background image
up everywhere but what is it? -- it’s a buffer with ions added because just water would not be efficient \TE = Tris + EDTACan we also make a google doc for the practical? describing basic techniques and what each person saw at the tables since she said she’d mix it up between both open lab days?Good luck y’all! Hope we all do amazing!Buena suerte a todos! Espero que nos vaya super chido!Sorte, eu espero que tudos tirem boa nota! bon chance je espère que nous le faisons tous bienQuestion: is there a reason that beta-mercaptoethanol was used as the reducing agent in 4x loading buffer while DTT is used as the reducing agent in the extraction buffer? rather than using one or the other for both solutions. Probably because beta-M is more volatile and it’s toxic so it shouldn’t be used with heat. Not sure if that’s why but I don’t see many other reasons. Yes, Beta-ME is more soluble and hence a more effective reducing agent; however, when we must heat our solution we switch to the less soluble (and less effective reducing agent) DTT as it is not volatile and so will not leave the solution when it is heated. If we kept only Beta-ME in the solution, when heated it would leave as a gas and we would have no reducing agent at all.6 Practical Stations:wild cardprobeDNASpecScope - does anyone have a good way of remembering how to set Kohler? Proteinmj mm8
Background image
Lab 7(Protein)Pre-Lab Questions1)Sodium azide (NaN3) is considered to be..a)a reagent toxic to all living things so extreme care with use and disposal should be taken. (Correct)b)to be nontoxic and may be safely discarded in the sink.c)a bio hazard that is infectious and needs to be autoclaved prior to disposald)a bacterial agent that should be most safely discarded in liquid bacterial wastee)a bacterial agent that should be most safely discarded in solid bacterial waste
Background image
Image of page 4

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture