Enzyme Assignment

Enzyme Assignment - Product Concentration (mM) Time (min)...

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Unformatted text preview: Product Concentration (mM) Time (min) Product Concentration (mM) 5 0.32 10 0.44 15 0.5 20 0.54 30 0.65 40 0.66 50 0.69 60 0.57 Graph A - Time Course 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 0 10 20 30 40 50 60 Incubation Time (min) SSC 0 0.02 0.03 0.07 0.1 0.13 0.27 0.67 PC 0.01 0.16 0.29 0.46 0.59 0.77 1.01 1.29 1.4 Product Concentration (mM) 1.2 1 0.8 0.6 0.4 0.2 0 0 Graph B - Substrate Concentration SSC - Substrate Concentration (mM) PC - Product Concentration (mM) 0.1 0.2 0.3 0.4 0.5 0.6 Substrate Concentration (mM) Temp 0 23 37 55 100 Product Concentration (mM) 0.1 0.31 0.35 0.44 0.36 Graph C - Temperature 0.5 0.45 0.4 0.35 0.3 0.25 0.2 0.15 0.1 0.05 0 0 20 40 60 80 100 Incubation Temp (Degrees Celsius) Product Concentration (mM) 0.2 0.15 0.1 0.05 0 0 20 40 60 80 100 Incubation Temp (Degrees Celsius) A. The product concentration values seem to peak after 30-50 minutes of incubation time. I believe that this time ra the highest value of product concentration. B. As the concentration of substrate increased the product concentration rapidly ascends within the first tenth of a m with that much velocity again. Enzymes can only work and form products for so long. Once an enzyme has created C. My results indicate that enzyme activity (ability to efficiently convert substrate into product) is directly related to enzyme was only able to create a product concentration of 0.104, while on the other hand the 55 degree celsius test tube had Product Con urse 40 50 60 70 min) centration 4 0.5 0.6 0.7 on (mM) ture 80 100 120 es Celsius) 80 100 120 es Celsius) believe that this time range is optimum for the enzyme to achieve hin the first tenth of a millimole. Afterwards, it levels off and never raises e an enzyme has created as much product as possible it becomes denatured. ct) is directly related to temperature. At a very chilly zero degrees celsius the gree celsius test tube had a concentration nearly five times as much at 0.442. ...
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This note was uploaded on 04/07/2008 for the course BI 211 taught by Professor Jones during the Fall '07 term at Southern Oregon.

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