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Name:
Section: AM
Lab Partner:
Total Points = 60 pts
Part I: Preparing Standard Solutions and a Calibration Curve
DATA, GRAPHS AND CALCULATIONS
1 pt
Concentration (M) Absorbance
1.25E05
0.12
2.50E05
0.28 3 pts
3.75E05
0.36
5.00E05
0.56
6.25E05
0.65
Purpose and Method
Part I: The purpose is to learn how to prepare standard solutions to generate a calibration curve.
Explain how this is accomplished in this experiment. What calculations do you need to perform?
What measurements will be made? [Do not provide experimental details here].
How do you go from calculated/measured values to accomplishing the stated purpose?
(
7 pts
)
The preparation of the standard solutions will be completed by performing some simpe calculations using the [M1V1=M2V2] formula. This
way we can get the volume of ferroin standards to give us the desired concentration. Using the Ferroin Stock solution we will be able to
make the desired concentration of ferroin standards. After we make the standards and place them into the cuvettes, we will then measure
the absorbancy of each of the 5 ferroin standards using a spectrophotometer. Once we have the values of absorbancy for each of the
different concentration of ferroin solution, we will plot an absorbance vs concentration graph and determine the linear regression line
using the excel program. This is the calibration curve that we were aiming to create from the data collected in part 1. The linear
regression line will be used to determine the concentration of a solution at a certain absorbance.
Part II: The purpose is to determine mg Fe in a
tablet.
Explain how this is accomplished in this experiment.
What measurements will be made? [Do not provide experimental details here].
How do you go from measured values (data) to calculated results to accomplishing the stated purpose?
Include relevant chemical equations and mathematical expressions in this discussion. (
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 Spring '08
 Chiu
 Chemistry

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