Lab Report 1 - EXCEL TABLES

Lab Report 1 - EXCEL TABLES - 6.5 Fragment 3 180 7.5 Cut...

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Table 1 : Expected and Observed Phenotypes of E. Coli with Plasmids Containing Spliced DNA on Ag Plasmid LB only LB / AMP LB / KAN LB / AMP-ARA Expected pAMP + / NO + / NO - / NO + / NO Expected pKAN + / NO - / NO + / NO - / NO Expected pGLO + / NO + / NO - / NO + / YES Unknown + / NO + / NO - / NO + / YES
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gars Pre-treated with Antibiotics ( GROW or GLOW?) Plus means growth, minus means no growth, Yes means th
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he culture glowed under UV light, and No means that it did not.
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Table 2 : Fragment Length and Relative Mobility After Electrophoresis in Both Lambda DNA and an Unknown C Fragment ID Fragment Length (bp) Relative Mobility (cm) (x axis on graph) (y axis on graph) Lambda # 1 23130 2.2 Lambda # 2 9416 2.5 Lambda # 3 6557 2.9 Lambda # 4 4361 3.4 Lambda # 5 2322 4.1 Lambda # 6 2027 4.3 Lambda # 7 564 6.4 Fragment 1 4500 3.1 Fragment 2 380
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Unformatted text preview: 6.5 Fragment 3 180 7.5 Cut DNA Sample, Both Cut With the HIN DIII Enzyme 10 1000 100000 1 2 3 4 5 6 7 f(x) = -1.15 ln(x) + 13.26 Relative Mobility of Lambda DNA vs. Fragment Length of DNA Column B Logarithmic Regression for Column B Fragment Length (bp) Centimeters Traveled 23130 2.2 9416 2.5 6557 2.9 4361 3.4 2322 4.1 2027 4.3 564 6.4 3.1 6.9 7.8 Table 3 : Expected Fragment Lengths of the Plasmid DNA Cuts by Restriction Enzymes Compared to the Unkn Plasmid Expected pAMP 3755 784 N/A N/A N/A Expected pKAN 2332 1875 N/A N/A N/A Expected pGLO 4496 399 226 220 30 Unknown 4500 380 180 N/A N/A ength of Fragment #1 (bp) ength of Fragment #2 (bp) ength of Fragment #3 (bp) ength of Fragment #4 (bp) ength of Fragment #5 (bp) nown Sample...
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Lab Report 1 - EXCEL TABLES - 6.5 Fragment 3 180 7.5 Cut...

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