Molec I & II Pre-Lab

Molec I & II Pre-Lab - Lauren Allen Monday at 6 Due January...

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Lauren Allen Monday at 6 Due January 22, 2007 1. The organism is E-coli and is a bacterium. 2. A plasmid is a small coiled ring of double-stranded DNA containing accessory genes of a bacterial genome. A plasmid is not a living cell and cannot grow, however a plasmid can confer the phenotype of glowing in the presence of UV light. It differs from chromosomal DNA in that it is shaped like a ring, can be transferred to other organisms (it’s a vector), and not all bacterium will contain plasmids. 3. This is called phenotypic identification because if a plasmid has conferred its DNA to a bacterial cell, it changes the phenotype. Using phenotypic selection, we can select for the new phenotype ensuring that the bacteria left on the plate has the transferred plasmid’s DNA. 4. If E. Coli had received the pAMP plasmid via transformation, it then would contain the ampicillin resistance genes and would then show the phenotype resistant to ampicillin. The cell therefore could grow on plates containing the antibiotic. 5. If the E. Coli cell had received the pKAN plasmid via transformation, it would then have the phenotype that is resistant to ampicillin and kanamycin. (It would also glow under UV light.) The cell could then grow and reproduce in both plates. 6. If the E. Coli cell had received the pGLO plasmid via transformation, it would then have the phenotype that’s resistant to the antibiotic ampicillin and that which glows under a UV light due to arabinose. The cell could then survive the
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This note was uploaded on 04/14/2008 for the course BIOSCI 0060 taught by Professor Ruthermund during the Spring '08 term at Pittsburgh.

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Molec I & II Pre-Lab - Lauren Allen Monday at 6 Due January...

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