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Virtual Bacteria ID Lab

Virtual Bacteria ID Lab - Lauren Allen Lab Monday 6-8:50...

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Lauren Allen Lab Monday 6-8:50 Virtual Bacteria Identification Lab 1. These traditional methods are less reliable than the newer methods because it is necessary to begin with a good sample of bacteria. Some bacteria do not grow on solid mediums (some grow only on liquid) and this makes it easier to distinguish between two closely related species. The newer methods allow even bacteria that grow poorly on a cultured medium to be identified. 2. To increase the number of bacterial cells received from the patient I would place the sample of bacteria on an agar plate with nutrients and give it time to grow. 3. The first step is to add a digestive enzyme buffer to the bacterial sample. This buffer eats away the cell wall of the bacteria. It may take a few hours. 4. The digestive enzymes are denatured by heating them in a hot water bath of 100 degrees Celsius. We must do this since we are using other enzymes in the next step. 5. We want the DNA (which is in the liquid portion versus the clump of cellular matter after centrifuging the tube). We remove the DNA / liquid contents via a micropipette. 6. PCR is a DNA cloning technique that allows for the duplication of DNA without the use of a living organism. 7. A double stranded DNA fragment is taken and heated until it reaches 95 degrees C. This produces single stranded fragments to which, when cooled, the primers and DNA polymerases can attach to and then copy. This makes it possible for a very large amount of DNA to be replicated in hours.
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