Copy of Copy of Amylase Enzyme- effects of pH

Copy of Copy of Amylase Enzyme- effects of pH - Trial #1...

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Unformatted text preview: Trial #1 pH4 pH5 pH6 Minutes pH4 0 3 6 pH5 0 3 6 pH6 0 3 6 0 min. 1.25 1.19 1.13 Starch Hydrolyzed 0 41 67.3 0 14.6 50 0 20.5 44.9 3 min. 0.67 0.9 0.72 6 min. 0.14 0.18 0.22 Standard Curve of [Starch] vs 1.2 1 Absorbance 580 nm 0.8 0.6 0.4 0.2 0 0 10 20 30 f(x) = 0.02x - 0.01 R = 1 Starch Concentrat Trial #2 pH4 pH5 pH6 pH7 Minutes pH4 0 3 6 pH5 0 3 6 pH6 0 3 6 pH7 0 3 6 [Starch] 0 12.5 25 37.5 50 0 min. 1.27 1.27 1.25 1.31 Starch Hydrolyzed 0 47.9 46.7 0 13.08 19 0 21.7 32.8 0 20.8 30.9 3 min. 0.3 1.01 0.81 0.89 6 min. 0.33 0.89 0.59 0.69 Starch Hydrolysis (mg/L) Starch Hydrolysis pH4 Trial 2 60 50 40 30 20 10 0 0 1 2 3 Time (min) 4 5 Starch Hydrolysis pH6 Trial 2 35 Starch Hydrolysis (mg/L) 30 25 20 15 10 5 0 0 1 2 3 Time (min) 4 5 Absorbance 0 0.23 0.5 0.74 1.01 Starch Hydrolysis (m 25 20 15 10 5 0 pH 4 Minutes 0 3 6 pH 5 Minutes 0 3 6 pH 6 Minutes 0 3 6 Trial 1 0 41 67.3 Trial 2 0 47.9 46.7 0 1 2 3 Time (min) 4 5 Starch Hydrolysis pH4 Trial 1 Trial 1 0 14.6 50 Trial 1 0 20.5 44.9 Trial 2 Starch Hydrolysis (mg/L) 80 70 60 50 40 30 20 10 0 0 1 2 3 Time (min) 4 5 0 13.08 19 Trial 2 0 20.8 30.9 Starch Hydrolysis pH6 Trial 1 50 45 Starch Hydrolysis (mg/L) 40 35 30 25 20 15 10 5 0 0 1 2 3 Time (min) 4 5 Starch Hydrolysis pH 80 Starch Hydrolysis (mg/L) 70 60 50 40 30 20 10 0 0 1 2 3 4 5 Time (min) Starch Hydrol 40 30 20 10 0 0 1 2 3 4 5 Time (min) Starch Hydrolysis pH 60 Starch Hydrolysis (mg/L) 50 40 30 20 10 0 0 1 2 3 4 5 Time (min) Starch Hydrolysis pH 50 45 Starch Hydrolysis (mg/L) 40 35 30 25 20 15 10 5 0 0 1 2 3 4 5 Time (min) d Curve of [Starch] vs Absorbance 580nm 20 30 40 50 60 Starch Concentration (mg/L) Hydrolysis pH4 Trial 2 20 18 Starch Hydrolysis (mg/L) 16 14 12 10 8 6 4 2 0 0 1 Starch Hydrolysis pH5 Trial 2 3 Time (min) 4 5 6 7 2 3 Time (min) 4 5 6 7 Hydrolysis pH6 Trial 2 35 Starch Hydrolysis (mg/L) 30 25 20 15 10 5 0 Starch Hydrolysis pH7 Trial 2 3 Time (min) 4 5 6 7 0 1 2 3 Time (min) 4 5 6 7 Starch Hydrolysis (m 25 20 15 10 5 0 3 Time (min) 4 5 6 7 0 1 2 3 Time (min) 4 5 6 7 h Hydrolysis pH4 Trial 1 60 Starch Hydrolysis (mg/L) 50 40 30 20 10 0 0 3 Time (min) 4 5 6 7 1 Starch Hydrolysis pH5 Trial 1 2 3 Time (min) 4 5 6 7 ch Hydrolysis pH6 Trial 1 2 3 Time (min) 4 5 6 7 Starch Hydrolysis pH 4 Column B Column C 3 4 5 6 7 Time (min) Column B Column C 3 4 5 6 7 Time (min) Starch Hydrolysis pH 5 Column B Column C 3 4 5 6 7 Time (min) Starch Hydrolysis pH 6 Column B Column C 2 3 4 5 6 7 Time (min) ...
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