manuscript review 2 - Effect of Salinity on Osmoregulatory...

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Effect of Salinity on Osmoregulatory Patch Epithelia in Gills of the Blue Crab Callinectes Sapidus Summary : Many studies have shown that the proportion of gill in blue crabs that is occupied by osmoregulatory cells (called a ‘patch’) is larger in crabs that are acclimated to dilute seawater than crabs that are acclimated to regular seawater. One goal of this study was to determine how much time is required for the size of the patch to increase. Another goal was to determine if there was a “dose-effect” with respect to salinity. That is, if lower salinities produced a larger patch than slightly higher salinities. The third objective the authors pursued was to determine whether existing gas transport cells differentiated into osmoregulatory cells (hypertrophy), or if existing osmoregulatory cells proliferated to increase the size of the patch. They also investigated the changes in hemolymph osmolality at lower salinities. To investigate these questions, the researchers maintained male “intermolt” blue crabs in tanks of seawater at 35 ppt for three weeks. After the crabs were acclimated to this salinity they were transferred to 10 ppt seawater. Samples of gill tissue were collected at regular intervals over the next 14 days. Three parameters were measured using different gill numbers from different sides of the body. These parameters were enzyme activity, relative patch area, and RNA and DNA content. Hemolymph osmolality was also measured. The results showed that the size of the patch did increase slowly after
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manuscript review 2 - Effect of Salinity on Osmoregulatory...

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