Guidelines for laboratory 3 post - -Calculate the V max for...

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Guidelines for laboratory 3 post-lab: Assay of Yeast Alcohol Dehydrogenase (ADH) Introduction: -Describe the reaction of the yeast alcohol dehydrogenase. -How do you monitor this reaction? -How can you determine the total enzyme concentration in this assay? -What information do you need to determine the total enzyme concentration? Results: -Show the raw data of your 4 assays: all absorbance values. -Show the rates for three enzyme assays.
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Unformatted text preview: -Calculate the V max for ADH for the three assays (formula on page 26).-Calculate the k cat from the SIGMA ADH spec sheet (in your enzymes lecture slides).-Use the kcat to calculate the total enzyme concentration. Discussion:-Explain what specific enzyme activity and turnover number tell you about the activity of an enzyme. What is the difference between the two?...
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