Unformatted text preview: -Calculate the V max for ADH for the three assays (formula on page 26).-Calculate the k cat from the SIGMA ADH spec sheet (in your enzymes lecture slides).-Use the kcat to calculate the total enzyme concentration. Discussion:-Explain what specific enzyme activity and turnover number tell you about the activity of an enzyme. What is the difference between the two?...
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- Winter '14
- total enzyme concentration, yeast alcohol dehydrogenase