Elucidating the Calvin Cycle

Elucidating the Calvin Cycle - Elucidating the Calvin Cycle...

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Elucidating the Calvin Cycle Throughout the 1940s and most of the 1950s Melvin Calvin and two post-doctoral researchers, Andrew Benson and  James Basham, developed several new techniques which were necessary in their quest to understand how plants convert  CO 2  into glucose. Things they needed: 1) A way to track movement of CO 2  as it changes into other chemicals:  14 C is a radioactive form of carbon.  In the 1940s,  radioactive isotopes first became available for research use.  Calvin et al. realized they could use radioactive C in CO 2. 2) A way to identify what chemicals were formed:  They developed two-dimensional paper chromatography.  Two different  solvents were used and run at right angles to each other.  This allowed separation of chemicals on the basis solubility in  butanol:water and in phenol.
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This note was uploaded on 04/14/2008 for the course BIOL 116 taught by Professor Meyer during the Spring '08 term at Austin College.

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