Light Microscopy Lab-2 - Light Microscopy Lab Write-up...

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Light Microscopy Lab Write-up Amber Elkins Dr. Bagavandoss September 22. 2007
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Introduction: The purpose of this experiment was to learn how to use the upright and inverted Olympus microscopes. Doing so, one should have gained an understanding of the relationship between the objective and ocular, have learned the essential techniques to prepare and observe cells, have observed and photographed a sample having utilized differed types of microscopy including various lens sizes, have learned to estimate the size of cheek cells in the micrographs and imported the images and have saved them for the laboratory report where the parts are then labeled. Materials & Methods: Supplies needed included one upright, one inverted Olympus microscope, methylene blue, onion skin epidermal cells, elodea plant leaf, human epithelial cells, four blank slides, four coverslips, and saline. Three different kinds of cells were obtained and made into slides. An onion skin’s epidermal cells were prepared by slicing a raw onion and cutting out a ring. The With forceps, the inner membrane was removed and placed on a slide. A drop of water was placed upon the graft, and
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  • Fall '07
  • Bagavandoss
  • cell biology, cell size, nucleus size, brightfield contrast

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