Chapter 14 Worksheet

Chapter 14 Worksheet - Chapter 14 RNA Molecules and...

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Chapter 14: RNA Molecules and Processing Important concept to remember: The coding sequence for a gene is not always continuous! In eukaryotes, segments of DNA that lie within a gene but are not translated into protein are called ___________________. These segments will be transcribed into pre-mRNA but will be spliced out during mRNA processing. The segments that are left in and are translated into protein are called ________________. Draw the basic structure of mRNA in prokaryotes. Make sure to label the three regions and don’t forget the start and stop codons and the Shine-Dalgarno sequence. In prokaryotes, as soon as transcription is complete, translation can begin. In eukaryotes the pre-mRNA is located in the _____________________ and translation is located in the ______________________. Therefore, eukaryotic mRNA must be processed and transported before translation. The three steps in eukaryotic mRNA processing are: 1. 2. 3. Addition of the 5’ cap What special nucleotide is used at the 5’ cap? ____________________________ Usually the phosphodiester bonds between nucleotides are 3’-5’ bonds. However, the 5’ cap has a special _______________ bond. (Hint: The guanine is put on “backwards”) List the four steps (in order) of the addition of the 5’ cap.
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