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Unformatted text preview: Page 1 Exam 2 Spring 2007 Biol 1362 Bubble in name (last name first). Bubble in last 4 digits of student ID # in PINK VERSION boxes G-H-I-J. Write color of exam above your name. 1. Which of the following are true of both eukaryotic and prokaryotic genes? A) In order to be transcribed, both require RNA polymerase to bind to a promoter. B) Transcription of both can be prevented by a regulatory (repressor) protein. C) Genes of similar function are organized and grouped under control of a single promoter. D) A, B, and, C are true. E) Only A and B are true. Ans: E 2. All are applicable to PCR except : A) It requires a DNA polymerase that is heat-resistant. B) It is used to amplify tiny quantities of DNA. C) It only replicates target sequences. D) It goes through temperature cycles of heating and coooling. E) There are no exceptions; A-D are all applicable to PCR. Ans: E 3. Choose the incorrectly matched pair: A) Post-translational control: prevent ribosome binding B) Post-transcriptional control: remove polyA tail C) Pre-transcriptional control: histone modification D) Transcriptional control: regulation of RNA polymerase activity Ans: A Use the following to answer question 4: The restriction enzyme HindIII recognizes the DNA sequence: A*A G C T T T T C G A*A Below is a DNA molecule: C T T C G T T A A G C T T C T T A A G C T T A C G A A G C T T T G A A G C A A T T C G A A G A A T T C G A A T G C T T C G A A A 4. How many fragments are generated by cutting the DNA molecule with the HindIII enzyme? A) 2 B) 3 C) 4 D) 5 E) 6 Ans: C 5. ____________ is a technique that can be used to separate DNA molecules on the basis of their size. A) Southern blotting B) PCR C) Gel electrophoresis D) Cloning E) Reverse transcription Ans: C 6. Which of the following are false concerning reproductive cloning? A) Involves the technique of nuclear transplantation B) The source of the donor DNA is the one who is cloned. C) Reproductive cloning is a very efficient process providing nearly 100% success in every attempt. D) It requires a surrogate mother in which to implant the embryo. E) B and C are both false statements. Ans: C 7. DNA microarray Ans: B) contains known sequences of all or most of the genes of a cell 8. gene therapy Ans: D) typically employs the use of viral vectors 9. Southern blot Ans: A) transfer of DNA fragments to a filter Page 2 10. tumor suppressor genes Ans: E) involved in the normal detection & repair of damaged DNA 11. oncogenes Ans: C) cancer causing forms of normal genes 12. Which pair is mis-matched ? A) prion:infectious protein B) animal virus infection:host could survive and produce viral progeny C) viroid:capsid D) reverse transcriptase:retrovirus E) conjugation:genetic transfer through contact between bacterial cells Ans: C 13. The following statements are false concerning exons of eukaryotic genes, except: A) Translation of exons in pre-mRNA occurs in the cytoplasm. Translation of exons in pre-mRNA occurs in the cytoplasm....
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