Lecture 18 Transcription pt-2slides

Lecture 18 Transcription pt-2slides - Readings Ch.13.1...

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13/03/2015 1 Readings: Ch.13.1, 13.2 -14.2 BIO1140 Winter 2015 Caroline Petit-Turcotte 1 Understand, describe and demonstrate the steps involved in transcribing DNA to mRNA Be familiar with the elements comprised in a transcription initiation complex and its role Explain and demonstrate RNA maturation and splicing Compare and contrast RNA transcription in prokaryotes vs eukaryotes BIO1140 Winter 2015 Caroline Petit-Turcotte 2
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13/03/2015 2 BIO1140 Winter 2015 Caroline Petit-Turcotte 3
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13/03/2015 3 DNA RNA Protein (insulin) What about prokaryotes? BIO1140 Winter 2015 Caroline Petit-Turcotte 6 Modify chromatin (histones) to reach DNA sequence In prokaryotes, DNA is directly transcribed to mRNA In eukaryotes, DNA is transcribed to a pre- messenger RNA, that will be matured into mRNA, and then be transported out of the nucleus
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13/03/2015 4 BIO1140 Winter 2015 Caroline Petit-Turcotte 7 The RNA strand produced is elongated in the 5’ to 3’ direction The RNA polymerase II reads the 3’ – 5’ DNA strand (the template strand)
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