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Unformatted text preview: the tube labeled -. 5. Incubate on ice for 15 minutes. 6. Label two LB Agar plates one as LB+ and the other as LB-. Label two LB/Amp Agar plates one as LB/Amp + and the other as LB Amp(-). Write your bench letter on each plate. 7. Heat-shock the cells at 42 C for 90 seconds. 8. Immediately return cells to ice for 2 minutes. 9. Add 200l of Luria broth to each rube. Mix gently by tapping it with your finger. 10. Incubate at 37 c for 45 minutes. 11. Place 70 l of + cells on center of LB(+) Agar plate and spread using a sterile L shaped glass rod. 12. Repeat step 11 on LB/Amp(-) 13. Allow plates to set for 5 minutes. 14. Tape plates for the thoe whole class together and label with TAs name. 15. Incubate inverted plates overnight at 37 C....
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- Spring '08