BIO lab rough1 - Jerry

BIO lab rough1 - Jerry - Crossing over in Prometaphase...

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Crossing –over in Prometaphase observed through Sordaria Fimicola  Jerry Jose March 3, 2008 Lon Ridgway  Abstract: The main purpose of conducting this Meiosis and Crossing-over in Sondaria Fimicola Laboratory  was to obtain a better understanding of how crossing-over takes place in Prometaphase of  Meiosis. The main reason for using the Fungus Sodaria Fimicola for this experiment was because  these funguses spend most of their life cycle in haploid condition; hence their growth meiotic cell  division is quite faster when compared to other species. Through this lab students observed the  hybrid crossing and color changes that occurred in the ascospores.  For this experiment a wild- type black (+) and tan (t) cultures of Sordaria were used.  These cultures were incubated for  seven days and were ruptured and observed under a microscope.  The class compared their  result in order to compare it with the results that were obtained by previous known facts about the  genetics of Sondaria.  Through the earlier known facts about the genetics of this species it was  hypothesized that a black color spore will be seen if both the genes were normal or wild-type and  that either of the genes had been mutated than the color would be tan or gray and if both the  genes were mutated that the spore would be colorless. Before we started this experiment we  hypothesis was to find 48% non-crossover and 52% crossover, but in reality we observed 47.7% 
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non-crossover and 52.2% crossover.  Our Conclusion was that the over null hypothesis was valid  since the degree of freedom is 1 and probability is .05 Introduction: There were several reasons that were connected with the overall decision to conduct this  experiment.  One of the major reason that I believe that we conducted this experiment was to gain  further knowledge and understanding of the cell division. Performing this experiment has enabled  me to gain hands on approach with the process of meiosis especially the late prophase where the  homologous chromosome exchanges genetic material through the process of cross-over.  One of  the other reasons was to compare and confirm my results obtained through my experiment with 
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This note was uploaded on 04/14/2008 for the course BIOL 1162 taught by Professor Anamedrano during the Spring '08 term at University of Houston.

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BIO lab rough1 - Jerry - Crossing over in Prometaphase...

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