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Biolab2report[2]part3[2] - Transformation of E coli cells...

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Transformation of E. coli cells using a Plasmid to Produce Ampicillin Resistance Simi Simon, March 23, 2007, Stephen Huff Abstract The purpose of this experiment is to identify whether or not the presence of the pAMP gene contributes to the resistance that E.coli (Escherichia coli) develops to ampicillin. In our experiment the cells of E. coli were formulated to recombine with the (plasmid) pAMP that contains a gene for ampicillin resistance, but in some cases the plasmid did not recombine. Other cells of the E. coli were given a T. E. Buffer. Then the cells were allowed to grow on the agar plates. My group’s hypothesis stated that if the Amp^r gene is transferred to Amp^s bacteria, then the recipient bacteria will be resistant to ampicillin. In the end the cells that survived and grew were transformed by the plasmid and the cells that died were not transformed. Introduction E. coli is very widely used organism in recombinant DNA research and the rest of the science world due to its ability to grow rapidly in most mediums (Helms 18-20). This experiment requires the usage of E. coli cells and the concept of transformation in cells. This experiment presents the idea of recombination. The information obtained from this experiment can have profounding affects on ampicillin resistance in our bodies. Results are needed from experiments like this to analyze new abnormalities that will come up in the future. The purpose of the experiment was to carry out the transfer of the pAMP to the E. coli cells. The hypothesis that was created for this experiment stated that if the Amp gene was to be transferred then the recipient bacteria would be resistant to ampicillin.
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This experiment is part of an extensive research called recombinant DNA research. Recombination of genes deals with three principles transformation, conjugation
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