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The Effects of Temperature and pH on Catalase Activity from Potato Enzymes Lab Report.docx

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The Effects of Temperature and pH on Catalase Activity from Potato Enzymes Lab ReportJessica Stabile25 October 2021
Introduction:The effects of temperature and pH on catalase enzyme concentration were determinedin this lab. In the previous lab, the catalase from potatoes were extracted and the catalaseenzyme concentration selected was 60% so the reaction was not too little or too strong. ThreepH solutions of 3, 7, and 11 were tested on the enzyme. Three temperatures (4 degrees, 25degrees, and 37 degrees Celsius) were tested on the enzyme. It is hypothesized that, enzymeactivity was the highest at 25 degrees Celsius and when the pH was 7 because they were boththe most neutral points. If an enzyme reaches above or below an optimum point oftemperature or pH for the enzyme the enzyme activity decreases or gets denatured.Materials and MethodsMaterialsEnzyme dilution determined by students during CAE1: 60%Seven test tubes/test tube rackpH solutions of 3, 7, and 113% hydrogen peroxideTimer

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Term
Fall
Professor
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Tags
Enzyme, pH, Incubation period, Catalase, Jessica Stabile

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