lab 7 - SYSTEMS BIOENGINEERING LAB II (Spring 2008) Lab #7:...

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. Page 1 . SYSTEMS BIOENGINEERING LAB II (Spring 2008) Lab #7: Nerve Conduction Instructor: Dr. Xiaoqin Wang Preparation: Observe compound action potentials (using 1 stimulus) In the following experiments, we will use frog’s sciatic nerve to explore physiological properties of action potentials. Set up stimulating and recording electrodes as shown below. In order to observe a bipolar compound action potential (CAP), the distal recording electrode should be away from the damaged end of the nerve. Suggested initial parameter settings: stimulus frequency (5-10Hz), duration (0.2ms), amplitude (0V). Slowly increase the stimulus amplitude until you begin to see a compound action potential (CAP) on the screen. Find out the maximum magnitude of CAP that you could generate. Change the placement of the recording electrodes and stimulus duration and/or amplitude to observe changes in CAP. Determine two stimulus conditions: (1) , where a CAP is evoked, (2) , at which a CAP reaches its maximum magnitude. Experiment 1. Measure amplitude-duration curve (using 1 stimulus) Your goal is to figure out combinations of stimulus amplitude and duration that barely evoke a CAP (i.e., at the CAP threshold). You need first to choose a response criterion ( Z ) to decide whether a CAP is evoked. For example, you may consider that a CAP is evoked when the waveform’s magnitude reaches 10% of the maximum CAP magnitude
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This note was uploaded on 04/15/2008 for the course BME 580.406 taught by Professor Wang during the Spring '08 term at Johns Hopkins.

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lab 7 - SYSTEMS BIOENGINEERING LAB II (Spring 2008) Lab #7:...

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