Genetics 1 - •Single nucleotide polymorphisms(SNPs...

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Genetics, October 20, 2014 Genome-  •The DNA content of a single cell. •Sequence organization •Genomics –  molecular mapping and characterization of whole genomes. •Bioinformatics •Comparative genomics •Functional genomics •Transcriptomes •Proteomes •Interactomes  •Whole-genome shotgun sequencing •Sequence randomly cloned fragments •Use a computer to look for sequence overlap •Ordered-clone sequencing •Create map by building contigs •Sequence the clones within the contingent   •Neutral polymorphic DNA markers •Single Nucleotide Polymorphisms (SNP) •Restriction Fragment Length Polymorphisms (RFLP) •Variable Number Tandem Repeats (VNTR), SSR •Minisatellite •Microsatellite
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Unformatted text preview: •Single nucleotide polymorphisms (SNPs) •Single base substitution that is polymorphic in the population. •3M in human genome, 1 every 300-1000 bases •Combinations of SNPs can be used to identify linkage •Haplotype = chromosome segment defined by an array of SNPs •HapMaps RFLP – restriction fragment length polymorphism •Minisatellites •15-100bp each •basis for original DNA fingerprinting •Digest DNA and use probe for repeat sequence •Offspring inherit bands from each parent •Microsatellite DNA •VNTRs of 2-4 nucleotides in length •DNA typing (paternity/forensics) •Use PCR and flanking primers...
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