Exam 3 - BIOLZBES Exam 3{Spring 2011 1 Itrill-rich ofthe...

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Unformatted text preview: BIOLZBES Exam 3 {Spring 2011} 1. Itrill-rich ofthe fflllD‘Wli'lQ is an example of a recombinant DNA molecule? A PCH-ampfrfied DNA fragment. Fl shtgle-stranded RNA hy'tn'idized to a single—stranded DNA. a. B. Q) A human cut-tat inserted into a bacterial plasmid: vector. D. A restriction fragment separated from other fitters in an agarose gel. E. A bacterial plasmid cut with a restriction enzyme. \e\ Which armies e. nor a [satire ofthe genetic code? It is used by ail organisms on earth {atthough minor exceptions in the usage of some { — A. \ oodons have been found]. \/r “31 it is nonovertaoping. Some amino article have n'qutiple cottons. H . Each amino acid is unambiguously specified bl; a singte cotton. “st-EEC it contains 3 different slop oodons. it. The HUC‘lIJflth test of Luria and DelbrElclr established that... _ ' . antibiotics trill most bacleria but induce resistance in some that Itwill sunrise. @ mutations occur as a result of random processes that can happen at anl,r time and anywhere in the genome. c. phages can infect bacteria. “So the mutation rate varies huge E. none of the above is cone-ct. Iy between different test tubes containing bacterial mitoses. K Sidney Brenner and Francis Crick used show thatthe genetic code is a triplet code. x.- \ @ back {retrersei \ s. run-rare / C. silent D. suppressor E. nonsense 5. Two DNA fragments are separated by gel electrophoresis. In what respect is traveled farther different from the other fragment? the fragment that U} A. It has higher AT content. B. it has sticky ends. '5. It carries a higher negative charge. " _ It is larger {more base pairs}. E It is srnfller [fewer base pairs). .- ‘Gfi Protein synthesis using an RNA template is called... I, A. transtonnation. ' t transduction. \ C: transcn'pliort. .r . translation. E. transcendence. BIDLEBES Exam 3 {Spring Zfl'l '1] 7'. Loss of the poly-A tail associaled with most eultaryo'lic mRNAs usually resutts in... . A. increased translation of the rnR NA. B. elongation otthe mRHA. V/ . initiation of reverse transcription by reverse transcaiptase. (if? destabilization and degradation ofthe mRNPL addition ofa new poer sequence to the mRNA. It. What dieting Lrishss a cDNA library from a genomic DNA Ebrary‘?l {El} cDHA library represents mRNAs produced in a particular tissue or group of tissues; genomic library represents all nuclear DNA of an organism. NB.“ ch'ilA library represents all nuclear DNA of an organism; genomic iibrary represent; 3/ mRNAs produced in a particulartissue or group of tissues. C CD NA lflJrary represents al transcription Iunits {hnfiHfisJ transcribed in a particular tissue; genomic library represents all nuclear DNA ofan organism. D . oDNA library represents all genes ofarl organism that are transcribed by reverse lranscriptase instead of RNA polymerase; genomic library represents all nuclear DNA of an organism. - a E. cDHA library represents all genes ot'an organism; genomic library represents all nuclear DNA of an organism. 9. What types of mutations are responsible for the change in meaning oi the wild-type sentence below? li'i 1:1 type: THE CAT SEE? THE DOG Mutation A: THE HAT EMT TEE DflG ionization B: THE HTS fi'i‘l‘l‘ HIED 06 J mtetioa C : TEE: GET SET RTE EDD G , A. frame shifts in all three r'rmtated sentences A: Inversion: B: Deletion; C: Insertion {9. A: substitution; B; Deletion; C: Insertion D. A: substitution: B: frame shift: C: subsiitulion “‘E,‘ A: Insertion: B: substitution: ID: Deletion 1D. Herman Muller diameter! the existence of mute-gens by Showing that x-rays induce lethal mutations in fruit flies. 1."'l|'l'|ich of the graphs strewn below illustrates best the relationship between the dose of radiation {if-aids} end the percentage of chromosomes were} that carried tether imitations alter radiation? iii IZKJZM (“'1 I. as ‘n/ r”\ U o“ BIDLESZEI- Exam 3 {Spring 2011} K The likely reason forthe replacement of uracil b‘j’ thymine in DNA is... "it. 13. 1-1. 18. A. that uracil can only term 2 hydrogen hands while thymine forms 3. " E. the resetting increase in the Stabililf of the genetic infonhation {spontaneous deamination at cytosine produces uracil] C. the resulting increase in the coding potential of the DNA. D. the resulting decrease in the melting temperature of DNA. LE) that one one ans would othenvise not he dilferent. The presence of which at the following components is gages-gig formtransiafion of the nucleotide sequence ofan mRNA into the amino acid sequence ofa protein? ‘—'u°i.. charged tRhlhe ‘5‘ AUG start codon “El-n a highly specific tFtNA For each ofthe 61 amino acid-specifying codons D. presence ofantioodo-ns in changed tRNAs that specificallyr bind to options in mRhlA ' all ofthe alcove x “J: The cotton for methionine is'iUG. What is the anticodon for this triplet in the RNA that can-ice: methionine? .5 11th Q 3’-UAC-5' E. 3'-G UA—fi’ C. S'AUG-fi' D. 3'-TAG-5' E. 3'—lu'lET—5' Which ofthe following would you expect to find in a riboaorne‘? -—.-'Ji.. only ribosomal Rhta. trRNA} El. snFthiPs C. pre-mRhlAs C? ribosomal proteins and IRMA, including the enztn'ne peptidyl transferase epiioosomes. incluiflng the enzyme pahrah polymerase AminmCfl—tfim synthetase Is the enzyme that ...... A. folds tRNA molecules into their proper configuration. adds the appropriate amino acid to an uncharged mm C. synthesizes IRMA from a DNA temptate. -D. causes IRMA molecules to bind to the arn'rnoacyi site or a nhosome. E. “gates one. Dogrnas are the hallmarks of totalitarian and religious ideologies. They have no place in science. but in the 1960‘s Francis Crick made an exception and fonnulalacl a “dogma" that is still uefid today and maintains that ...... . genetic information is contained in both DNA and protein. @ genetic information one only.r flow from DNA {nucleic acid] to protein. E the lioinI of genetic information is bidirectional {from DNA to protein and vice verse}. D. genetic information can flow from DNA to RNA and tries verse. E. molecular biology is superiorto all other sciences. i were slit—“i 9mm 1?. or” J BIDLESZS Exam 3 {Spring 2011} The nucleolus is A. a small cell nucleus ["nehenttem") beside the rrrejor cell nucleus. E) a structure in the sell nucleus consisting of ribosome precursors. C. a mulliprotein complex responsible for splicing of the pro—mF-th. B. only visible in the meteohase of mitosis. E. the cell organelle at which protein biosy'nlhesis {translation} takes plane. in 19-54, Hironberg and Leder used the triplet-binding assay to determine specific oo-oon assignments. A eompie: of which ofthe following oompments was trapped in the nitrocellulose filter? A. ribosomes and DNA B. free tRan uncharged tRMAa and ribosomes C. (3) charged EMA, RMAu'iptet, and I'ihesotne sense and enfisense strands of DNA fir Assume that a new low-midrie sweetener is developed. The sthcture is novel and: is tested with the Ames test for muiagenicityt The following results are obtained: Sample Numb-er ofhisr revertant oodonies distitled water 2 distilled water + rat Ever enzymes 3 sweetener in distilled water 95 sweetener + rat liver enzymes in distilled water 93 What mammalian is most mrtsisient Iniith this data? A. The sweetener is HGT rnu Legenie. t—B. The sweetener IS mot-agents. The sweetener is not rnutagenic but can be mmened into strong mutagen. . Hat litter enzymes are highly mutagenie. E. Rat liner enzymes oontrert sweetener Into non-mutagenic compound. fit. The Sheep structure of eukaryotic mRMhs is of peniglar irriporlsnoe for "w 2‘1 . J 5A. mRHA transport. Et. p-olyadenylation. 9;] transcription. En. etfioient iransiation. E. none of the above. Mutations listed below mayI interfere with proper expression of an affected gene tenor-eaten defined as production of normal amounts ofa functional protein}. Which of the mutations is roost likely to have the W negative effect? .. t.__ A single nucleotide insertion in the DRF [open receding frame} near its 3'—eni:l. it? is single nudeefide deletion in the oer topsn reading frame) near its trend. iii A deletion of 3 onnseoitiirs nucleotides in the middte of the one A single base exchange at position -5EI in the promoter. -— A mutation that transforms the stop notion in an amino acid—encodmg codon. 5 BIDLEEEE Exam 3 {Spring 2011} it Which of the following statements is correct? A. Promoter regions are tlenstl‘ihed. out located upstream of the open reading frame {DEF}. whereas the 3'-LtTR of a gene is not transctihed. but oonmins signals that stop banscr‘iption. \fi. Promoter regions are transmitted and therefore located downstream of the transcription start. whereas the 5'-UTR of a gene is located upstream of the promoter forming the V; 5'-tenninal region of a gene. “(9 Promoter regions are not transcribed and located upstream ofthe transcription start. 'ttfltEt'EElE the 3'-UTR of a gene is transcribed. located downstream of the transcription start. yet contains no information for the amino acid sequence ofa protein. " D. Eul-taryotio genes usually contain 'n'itrons that are spliced out ofthe gone before transcription. whereas the promoter region remains in the gene. because it is the binding site of RNA pehrITbaIeae. E. The E'JJTR is also referred to as the “leader”, because it immediately precedes the first eaten of a gene. Irtlhereas the combined exons and introns are referred in as a "transm'iption unit“. because they constitute the onlyI region of the gene that is transcribed. 23. Splicosontes consist of..." A. specific proteins required for RNA splicing. ._ Et. specific RMAs required for RNA splicing (entities). C. ribosome summits. (gt: A and a. .’ None of the above. but... During translation in a prokarpotic cell, which shucbsre is of particular importance for initial 'r tint-ting ofthie mRNA to the small meme subunit and recognition oi the AUG stan option? _ W. ..F A. Poly A Shine—Dalgamo boot ‘5 TATA box. Promoter 25. A team ofscientists studying autism in humans cloned a gene suspected to be Iinlted to the disease. The gene encodes a protein offiflt amino acids. What is the length in nucleotides of the gain-coding part texons] of this gene? at we (E? 2043 V. 631 L p ”i1“ [3. £122”? E. Cannot be predicted. because the number of introns is unknown. 2E. Which oftt'te following is an essential feature of plasmids that are used as cloni'tg vectors? A. origin of replication B. oer-tremors . I2. tetomeres D. gene encoding a protein that acts as an antibiotic ’ Q2), all of the abet-re 2?. x2“ .1:- 28. 2'9. BIDL2323 Exam 3 {Spring 2011) Below you aee the data of aecuencing reactions according to Sanger {chain termination method}. What is the sequence efthe Eel—ate strand? aecaaoccra-a' G A T C — A. B. 5'-ATCGGMGG-3' 5‘-CGTT¢CGAT-3' ’—TAGEC'.I'I'GC-3' . 5‘-GGGAMTCC—3' _.-- @C AfiGtCflCl—fl One Iareli-eslaIzilischaai function at introns is to.._... a increaae the coding potential at a gene by allowing for altemati'ue splicing. '-. encode the amino acid sequence at proteins. — C. signal the end of transcription {terminator}. D. mediate the pairing of Mmohgoua chromosome-a during meiciaia. E. hind RNA polymerase. Which thhe following are ecn'u'r-cn pcatlranslalionai n'lc-dificarlions that regulate die activity of many proteins ...... Phesphoryleticn and daphcaphcrylation of specific amino acid side chains . Camcxylation and decarboxyleflon of specific amino acid side chains _ I3. Hydroxylalicn and dehydrorylation of specific amine acid aide ehaine Er. Cleavage ofspecific amino acid aide chains. E. None of the above $ilent mutaticna ..... GE.) are null mutations El. lead to a single conservative amino acid exchange. H—C. do not change the primary atmdure {amino acid sequence} ofthe encoded protein. D. are recessive and, therefore, only cause a mutant phenatype when present in two copies. E. lead to deafness. BIDL2323 Exam 3 {Spring 20‘: 1} "#1 Shown are the reoognition sequenoea and cut sites of three reshtction uei-Lzymee. P- piaismid is c utwith $al'l. and combined wilh genomic one. digested with Xhoi. In plasmids with a genomic fragment. the Sell-Xhol junction can be outwith: I‘r/ both Xhol and Salli _ treat one '[1 C. Soil on'ry D. all three enzymes E. Teal onty 32. Wish ofthe lotion-ring is true for prolraryotes, butfltffll‘ eukaryotes? A. B. J c. D. “'E. Genes usualiy contain introrts and axons. Transatption and translation take place in diflerent cellular compartments. muss are usually potyadenylated. Transcription or genes that encode a '5 only one type of RNA polymerase. None ofthe above. Xhol 5 '... CEEG.H3 ' 3"..GAG 5' 5311 5 ' "JG EACHE ' 3 * HERE _,5 ' Ta qt 5'...1 ISA—3" 3I__A __5r roteins and functional FEMAe [TRMFL {RNA} requires #' How do bacteria protect their own DNA from restriction eruyrnes they possess? so. of as r\_ enzyme. By surrounding their DNA with By not having the restrictions e Ely d'len'lioal modification of the Ely intruding of represses proteins to the reoogn'rti They do not protect that used for genetic recomb Which offlte toner-ring can sense We mutations? A. Errors in DNA replication El. Hydrolysis oflhe glyoosidir: bond between base and deoxyribose . Reaction wilh H90 that deeminates bases All: of the above . Only E5 and G The key sent-me used in FER (polymerase chain reaction} is A. Tao polymerase. C. RNA polymerase. D. lehsmerase. E. Iigase. — reverse transu'iptaae. a men-threats ttbat is impassable to the reslriction enzyme. W reecgnition sites in their DNA. recognition sites that is carried out by a DNA methytase. on sites that block aces-as of the restriction r DNA. because sites reoognized by the lash-iction enzymes are inatien that would otherwise be suppressed. |.-' ...
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