Biology 118 Enzyme Lab Calculations

Biology 118 Enzyme Lab Calculations - y = 0.002x 0.0004 y =...

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Biology 118 Enzyme Lab Calculations: D1. Convert absorbance values to dopachrome concentrations. Subtract absorbances of the control tube from the assay tube. Corrected Absorbance Data: Test Tube 6 (1 min) - Test Tube 5 (1 min) = 0.151-0.062= 0.089 = 0.089 D1 Part 2: y = 0.3104x 0.035 / 0.3104 = x x = 0.11 mM D2: Convert the Dopachrome concentration (mM) into an amount of Dopachrome in millimoles using your final volume of the reaction tube. (1 pt.) mM x L = millimoles 0.0061 mM x 0.005 = 0.00031 mmol D3: In the excel template, graph each amount of Dopachrome vs Time. Each Dilution should be a separate line with its own equation, superimposed on same axes. Clearly label each line. If the trend for a line does not appear to be linear, discuss with your Grad TA what to do. You may state the outliers (which dilution, which time) and consider calculating the R-squared with and without the outlier. Again, best to discuss with YOUR Grad TA (4 pts.) (Shown on graph) D4: Calculate the Vi using the linear equation from D3. Note that the trend must be linear to do so. (2 pts.)
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Unformatted text preview: y = 0.002x - 0.0004 y = 0.002(4.0) - 0.0004 y=0.0076 D5. In the excel template, graph Vi vs Dilution for all Dilutions. Make note of any outliers, and discuss how to proceed with Grad TA. Include R-squared (3 pts.) (Shown on graph) D6. Determine the enzyme activity for the reaction. Activity = Product produced / time / volume. Include proper units! (2 pts.) Activity = Product produced / time / volume Activity = 1.90 mol/4 min/5 mL Activity = 0.095 or 0.1 mol/min-1/ml-1 D7. Determine the enzyme activity in the undiluted potato extract. Average the different values for all 4 dilutions. (2 pts)NOTE: The values for each dilution should be very similar and are measured per unit volume (ie, mmol/min/ml) Activity = 1.90 mol / (rate of reaction x volume) Activity = 1.90 mol / (0.0006 m/s x 5 mL) Activity = 3.17 mmol/min/ml Average: 3.1700 mmol/min/ml + 16.5000 mmol/min/ml + 1.8900 mmol/min/ml + 31.7000 mmol/min/ml / 4 = 13.2000 mmol/min/ml D8. Convert activity /volume to activity /weight (2 pts) 13.2 / (1/13.2) / 3750 = 0.05 mmol/min/mL...
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