Biology 118 Enzyme Lab Calculations

# Biology 118 Enzyme Lab Calculations - y = 0.002x 0.0004 y =...

This preview shows pages 1–2. Sign up to view the full content.

Biology 118 Enzyme Lab Calculations: D1. Convert absorbance values to dopachrome concentrations. Subtract absorbances of the control tube from the assay tube. Corrected Absorbance Data: Test Tube 6 (1 min) - Test Tube 5 (1 min) = 0.151-0.062= 0.089 = 0.089 D1 Part 2: y = 0.3104x 0.035 / 0.3104 = x x = 0.11 mM D2: Convert the Dopachrome concentration (mM) into an amount of Dopachrome in millimoles using your final volume of the reaction tube. (1 pt.) mM x L = millimoles 0.0061 mM x 0.005 = 0.00031 mmol D3: In the excel template, graph each amount of Dopachrome vs Time. Each Dilution should be a separate line with its own equation, superimposed on same axes. Clearly label each line. If the trend for a line does not appear to be linear, discuss with your Grad TA what to do. You may state the outliers (which dilution, which time) and consider calculating the R-squared with and without the outlier. Again, best to discuss with YOUR Grad TA (4 pts.) (Shown on graph) D4: Calculate the Vi using the linear equation from D3. Note that the trend must be linear to do so. (2 pts.)

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: y = 0.002x - 0.0004 y = 0.002(4.0) - 0.0004 y=0.0076 D5. In the excel template, graph Vi vs Dilution for all Dilutions. Make note of any outliers, and discuss how to proceed with Grad TA. Include R-squared (3 pts.) (Shown on graph) D6. Determine the enzyme activity for the reaction. Activity = Product produced / time / volume. Include proper units! (2 pts.) Activity = Product produced / time / volume Activity = 1.90 mol/4 min/5 mL Activity = 0.095 or 0.1 mol/min-1/ml-1 D7. Determine the enzyme activity in the undiluted potato extract. Average the different values for all 4 dilutions. (2 pts)NOTE: The values for each dilution should be very similar and are measured per unit volume (ie, mmol/min/ml) Activity = 1.90 mol / (rate of reaction x volume) Activity = 1.90 mol / (0.0006 m/s x 5 mL) Activity = 3.17 mmol/min/ml Average: 3.1700 mmol/min/ml + 16.5000 mmol/min/ml + 1.8900 mmol/min/ml + 31.7000 mmol/min/ml / 4 = 13.2000 mmol/min/ml D8. Convert activity /volume to activity /weight (2 pts) 13.2 / (1/13.2) / 3750 = 0.05 mmol/min/mL...
View Full Document

{[ snackBarMessage ]}

### What students are saying

• As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

Kiran Temple University Fox School of Business ‘17, Course Hero Intern

• I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

Dana University of Pennsylvania ‘17, Course Hero Intern

• The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

Jill Tulane University ‘16, Course Hero Intern