PoppingPlasmidsStudentInstructionSheet

PoppingPlasmidsStudentInstructionSheet - Popping Plasmids...

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“Popping Plasmids” Instruction Sheet by Alexandra Leslie, Undergrad TA, Bio 118 Definitions : Plasmid : Small circular DNA in bacteria (bacT). Plasmids contain DNA that is independent of the bacT’s main chromosomes. Contains an origin of replication, so it is capable of self-replication. Transformation : In the lab, this is the introduction of new DNA into cells by causing cells to absorb DNA from their environment. Plasmids serve as vector for this. Plasmids can be introduced into bacT cells somewhat successfully, and one can determine whether the transformation is successful by then innoculating the bacT cells onto special agar nutrient plates that contain antibiotics, selecting for the transformed cells (only the transformed cells will survive). Why? For example, if you wish to introduce a gene into bacT, this can be done by including the gene on a plasmid which also contains a gene conferring resistance to the antibiotic ampicillin (amp R gene). When plated on culture media containing ampicillin, only those bacT which were successfully transformed (now have the plasmid) will survive.
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