5 - Kuo, 580.221 16 Sept 2004 Chirality; Conformations...

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Kuo, 580.221 16 Sept 2004 1 Molecules & Cells BME 580.221 Simple vs Complex Binding: Myoglobin vs Hemoglobin Lecture #05, 16 Sep 2004 Scot C. Kuo 16 Sept 2004 Kuo, 580.221 Chirality; Conformations & Covalent bonds – Chirality (e.g. left-hand vs. right-hand) • Definition : Without breaking bonds, no way to reorient molecules of different chirality to super-impose identically with each other. • Only certain atoms are chiral (i.e. ‘chiral centers’). – Must be 4 bond directions (tetrahedral), or more (metal bonds) – Different things in at least 4 directions. • Example: L- vs. D- amino acids – Freedom of single-bond to rotate • Conformations: no change in bonds, just rotate around them • Peptide bond ‘dihedral’ angles (angle formed by geometric planes) 16 Sept 2004 Kuo, 580.221 Concepts/Vocabulary Binding reactions: • Fractional Occupancy (θ) & Binding curves – Sigmoidal (=‘S’ shaped); Hyperbolic – Biology: FracOcc ( θ ) is more useful than moles • Multivalent, Independent (Antibody) • Multivalent, Cooperative (Hemoglobin) – Story: Heme group, metals, pH • Hill coefficient, n . 16 Sept 2004 Kuo, 580.221 Univalent binding • Fractional occupancy (only a few receptors/DNA sites, etc) • Binding curves; Linearization – Hyperbolic (linear axes)
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This note was uploaded on 04/15/2008 for the course BME 580.221 taught by Professor Dr.vidal during the Fall '08 term at Johns Hopkins.

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5 - Kuo, 580.221 16 Sept 2004 Chirality; Conformations...

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