7-notes - 580.221 BME Molecules and Cells L07 Dogma of...

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580.221 BME Molecules and Cells L07 Dogma of MolBiol 2 p 1 Lecture Seven – RNA: Transcription and Translation Outline: Review Central Dogma of Molecular Biology Detailed Mechanism of Translation Overview: Prokaryotes : DNA => mRNA => protein Eukaryotes : DNA => hnRNA => mRNA => protein hnRNA = hetronuclear RNA mRNA = mature or messenger RNA tRNA = translational RNA rRNA = ribosomal RNA Prokaryotic Transcription Contiguous genes (no introns) [picture] One control region – multiple genes. Each mRNA may have more than one gene, thus more than one ribosomal binding sequence (Shine-Delgarno). Eukaryotic Transcription Non-contiguous genes (introns & exons) One control region – one gene. RNA polymerase makes hnRNA, which includes copies of both introns and exons. RNA is not mature and ready to leave nucleus until : Splicing (removal of introns) 5’cap is added polyadenylation The polyadenylation of all mRNA makes their isolation simple – a gold bead with a short poly-T oligonucleotide will capture any and all mRNA in eukaryotic cells. How does RNA code for protein?
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This note was uploaded on 04/15/2008 for the course BME 580.221 taught by Professor Dr.vidal during the Fall '08 term at Johns Hopkins.

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7-notes - 580.221 BME Molecules and Cells L07 Dogma of...

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