8 - Kuo, 580.221 28 Sept 2004 1 Molecules & Cells BME...

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Unformatted text preview: Kuo, 580.221 28 Sept 2004 1 Molecules & Cells BME 580.221 Gene Regulation Lecture #08, 28 Sept 2004 Scot C. Kuo 28 Sept 2004 Kuo, 580.221 Concepts/Vocabulary • Genetics – trans and cis complementation • Transcriptional regulation (bacteria: lac operon: promoter, operator) • Splicing: intron & exon • Enhancer, TATA box 28 Sept 2004 Kuo, 580.221 Central Dogma of Molecular Biology • DNA->RNA->protein • All cells, from bacteria to human, express genetic information this way. 28 Sept 2004 Kuo, 580.221 How to regulate gene expression? • Importance of genetics: fast, but indirect testing • Choose to study an “inducible” system (can use environment to turn genes on/off) • Moving from genetics to molecular-level understanding 28 Sept 2004 Kuo, 580.221 Importance of Genetic Analysis • Mutations in genes that encode proteins • Mutations in regulatory regions around genes (promoters, etc.) • Mutations in genes that encode regulatory proteins that bind regulatory regions 28 Sept 2004 Kuo, 580.221 Types of Mutations Kuo, 580.221 28 Sept 2004 2 28 Sept 2004 Kuo, 580.221 Reminder about genetics • Gene/chromosome copies – Haploid : single set of chromosomes (e.g. bacteria and many unicellular organisms) – Diploid : two copies of each chromosome – Plants, cancer cells, and some fruit fly cells have more copies • Diploid genetics: – Genotype : Homozygous (identical “alleles”) vs. heterozygous (different alleles) for a gene – Phenotype : Recessive...
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This note was uploaded on 04/15/2008 for the course BME 580.221 taught by Professor Dr.vidal during the Fall '08 term at Johns Hopkins.

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8 - Kuo, 580.221 28 Sept 2004 1 Molecules & Cells BME...

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