11 - Kuo, 580.221 12 Oct 2006 Molecules & Cells BME...

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Kuo, 580.221 12 Oct 2006 1 BME 580.221 Antibody Isotype Switching Cellular Architecture Protein Trafficking Lecture #11, 12 Oct 2006 Scot C. Kuo 12 Oct 2006 Kuo, 580.221 Concepts/Vocabulary • Antibody Isotypes: isotype switching (DNA recomb., different from VDJ recomb.) • Cellular Receptors: – Review: cell structure, lipid bilayers – Methods to visualize parts of cells – Testing methods: protein gels (anim.), protection from proteases, Western blots (anim.) 12 Oct 2006 Kuo, 580.221 Clonal Selection Hypothesis (1950’s) • Ab specificity determined by amino acid sequence • Each Ab-producing cell produces one Ab specificity (specificity pre-exists encountering Ag) • Ab “displayed” on surface of cells • Binding of Ag to cells stimulates cell growth (proliferation) and Ab secretion • Self-Ab are eliminated early in life • Clones persist after disappearance of antigen (memory) L04-05 L10-11 L12 Themes driving this course: 12 Oct 2006 Kuo, 580.221 Germline Organization Light Chain: V+J+C Heavy Chain: V+D+J+C: C µ , C δ , C γ , C α , and C ε code for IgM, IgD, IgG, IgA, and IgE (each Cx has multiple exons) 12 Oct 2006 Kuo, 580.221 Ig Recombination 12 Oct 2006 Kuo, 580.221 Structure of Ig Classes (isotypes) How are the different isotypes generated?
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Kuo, 580.221 12 Oct 2006 2 12 Oct 2006 Kuo, 580.221 Germline organization of isotype constant regions 12 Oct 2006 Kuo, 580.221 Isotype Switching (beyond D) 12 Oct 2006 Kuo, 580.221 Isotype switching is not like VDJ recombination All isotype switch recombination is productive (i.e. no bad/non-expressed results) • Isotype switching uses different recombination signal sequences and enzymes • Isotype switching occurs after antigen stimulation (not during B-cell development in bone marrow) • Isotype switching is not random, but is regulated by T-cells 12 Oct 2006
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This note was uploaded on 04/15/2008 for the course BME 580.221 taught by Professor Dr.vidal during the Fall '08 term at Johns Hopkins.

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11 - Kuo, 580.221 12 Oct 2006 Molecules & Cells BME...

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