12 - Kuo, 580.221 17 Oct 2006 Molecules & Cells BME...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
Kuo, 580.221 17 Oct 2006 1 BME 580.221 Protein Secretion/Trafficking Lecture #12, 17 Oct 2006 Scot C. Kuo 17 Oct 2006 Kuo, 580.221 How to study protein trafficking 17 Oct 2006 Kuo, 580.221 Which membrane pathways leads to the outside of the cell? • Endoplasmic reticulum and Golgi bodies • Are these membranes like mitochondria? 17 Oct 2006 Kuo, 580.221 17 Oct 2006 Kuo, 580.221 Free vs Membrane Bound Ribosomes 17 Oct 2006 Kuo, 580.221 Expt: lumenal location of secretory proteins Tools : proteases, detergents, protein gels (polyacrylamide electrophoresis & SDS; Westerns) >Show Flash Animations< Microsomes are biochemically purified versions of endoplasmic reticulum
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Kuo, 580.221 17 Oct 2006 2 17 Oct 2006 Kuo, 580.221 Expt: Evidence for CoTranslational Insertion 17 Oct 2006 Kuo, 580.221 Crossing the ER membrane 17 Oct 2006 Kuo, 580.221 Translocation of soluble protein 17 Oct 2006 Kuo, 580.221 Mito. vs ER protein processing Yes -- many ?? Translation req’d for translocation SRP, then SRP receptor on ER ER no no Complete protein will translocate receptor on Mito Mito Add’l trafficking Add’l Protein Modifications Protein Signal Seq Binding 17 Oct 2006 Kuo, 580.221 Translocation of soluble protein (Ab?) 17 Oct 2006 Kuo, 580.221 Integration of transmembrane protein
Background image of page 2
Kuo, 580.221 17 Oct 2006 3 17 Oct 2006 Kuo, 580.221 Integration of double-pass transmembrane protein 17 Oct 2006 Kuo, 580.221 Questions for Ab secretion How to assemble? 4 polypeptides multiple disulfide bonds between peptides addition of carbohydrate (sugars) to peptide (common feature of secreted proteins; why?) How to move from ER to outside cell? How to get membrane receptor form?
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/15/2008 for the course BME 580.221 taught by Professor Dr.vidal during the Fall '08 term at Johns Hopkins.

Page1 / 9

12 - Kuo, 580.221 17 Oct 2006 Molecules &amp; Cells BME...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online