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Unformatted text preview: Methods that scientists use to copy dna from one cell (human) • Cut dna and paste it into another organism • You’re a scientist and you want to study the protein x from humans… • PCR copy of DNA • The polymerase chain reaction (PCR) can be used to quickly copy any DNA • To clone a gene you first perform PCR • An enzymatic reaction what enzyme makes dna from dna? Dna polymerase • Mixture exposed to cycles of heating to separate the dna strands • During each cycle , dna replicates, doubling the amount • 1,2,4,8, etc. • After 30 cycles of PCR, yo would have over 1 million DNA that were identical to the original version. Advance uses of PCR Use it to mutate a gene Put genes back into organism, see what happens. Restriction Enzymes Enzymes are used to cut and paste dna Dna from two sources cut ny restriction enzymes at specific restriction sites Resulting restriction fragments...
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- Spring '07
- DNA, Recombinant DNA- DNA