Lung lab pre-lab instructions

Lung lab pre-lab instructions - cylinder for an accurate...

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Lung lab 03-04-2015 Abstract Introduction Materials The materials used in this lab were: one liter of Deer Park water, a Fleaker for urine collection, a 100 ml graduated cylinder, micropipettes, spectrophotometer, micro test tubes, distilled water, parafilm, stopwatch, and Infinity™ Creatinine Liquid Stable Reagent. The test subject was a 30 year old male in good physical shape, with no past history of mental health problems or chronic disease. Methods In preparation for this lab the restrome was visited and the bladder was emptied. The test subject then drank a one liter bottle of deer park water in the shortest amount of time possible. Once all the Deer Park water had been drunk the stop watch was started. After a period of 30 minutes the test subject returned to the rest room and emptied his bladder into the Fleaker. The stop watch was reset and the time for the second collection was started. During the next 30 minute waiting period, the urine was transferred from the fleaker to a graduated
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Unformatted text preview: cylinder for an accurate measurement. 10 microliters of urin was then removed using a micropipette and placed in a micro test tube. 90 microleters of distilled water was then added to the tube to create a 1:10 dilution of the original urine. A 60 microliter sample of this dilution was then placed in a cuvette and 600 microliters of the assay solution, Infinity™ Creatinine Liquid Stable Reagent, was added to the cuvette. The cuvette was covered with parafilm and inverted to mix. The cuvet was then placed in a spectrophotometer that had been previously blanked using Results and data Discussion Supplemental Material References 1. Krieger, Paul A. A Visual Analogy Guide to Human Anatomy & Physiology / Paul A. Krieger. Englewood, Col.: Morton, 2013. Print. 2. Silverthorn, Dee Unglaub, Bruce R. Johnson, William C. Ober, Claire W. Garrison, and Andrew C. Silverthorn. Human Physiology: An Integrated Approach . Boston: Pearson Education, 2013. Print....
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