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Unformatted text preview: To develop tailored therapies, specific to each patient and disease, based upon individual genetic makeup and markers. 3. (30 pts) Briefly outline the steps of organismal cloning. (Use Dolly the sheep as an example.) 1. Donor sheeps mammary cell is extracted and grown in a tissue culture flask. Another sheeps unfertilized egg is extracted and the nucleus is removed. 2. The enucleated egg is combined with the nucleus of the cell to be cloned. 3. Fusion and division is stimulated by electrical pulses. This initiates development of the egg into an embryo. 4. The embryo is transferred into a surrogate ewe. 5. The pregnancy proceeds and the lamb is born via a C-section which is a clone of the original donor nucleus....
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- Fall '08