WEEK 15. INOCULATION.docx - BACHELOR OF SCIENCE IN...

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BACHELOR OF SCIENCE IN ENVIRONMENTAL SCIENCE:ENVIRONMENTAL MICROBIOLOGYCOURSE MODULECOURSE UNITWEEK151515INOCULATION OF CULTURE MEDIAReadcourseand unit objectivesRead study guide prior to class attendanceRead required learning resources; refer to unitterminologies for jargonsProactively participate in classroom discussionsParticipate in weekly discussion board (Canvas)At the end of this exercise, each student must be able to:Discuss the rationale of aseptic techniqueLearn how to aseptically transfer bacteria from one form of culture medium to anotherLearn the different inoculation and isolation techniquesINSTRUCTIONSStudy how to aseptically transfer bacteria from one medium to another.• Study the different inoculation and isolation techniques.• Watch the video tutorial on aseptic technique and inoculation methodso ASEPTIC TECHNIQUEo STREAK PLATE TECHNIQUEo INOCULATION OF SLANT TUBE, BROTH AND AGAR DEEPo POUR PLATE METHODo o SPREAD PLATE METHODo INOCULATION FROM BROTH TO BROTH, BROTH TO SLANT, BROTH TO PLATE
• Accomplish Lab Report # 15INTRODUCTIONBacteria were first observed by Anton von Leeuwenhoek in the late 17th century, but didn’tbecome the objects of serious scientific study until the 19th century, when it became apparentthat some species caused human disease. With the evolution of antibiotic-resistant strainsmedical researchers have refocused their attention on disease-causing bacteria and arelooking for new ways to defeat them. Scientists grow bacteria in pure cultures for this purpose.A population of bacteria grown in the laboratory is referred to as a culture. A pure culturecontains only one single type; a mixed culture contains two or more different bacteria.Isolation of bacteria must be performed aseptically, to ensure that unwanted bacterial or fungalcontamination is kept out of an important culture.In microbiology, aseptic techniques essentially require good laboratory skills. To maintain anaseptic work environment, everything should be initially free of microbes. Thus, it is importantto begin with pre-sterilized pipettes, culture tubes, and glassware. Inoculating loops andneedles made of metal wire can be used to transfer bacteria from one medium to another,such as from the surface of an agar plate to a broth. Metal tools may be sterilized by heatingthem in the flame of a Bunsen burner. Glass tools or metal spreaders or forceps that can’t besterilized by direct heat are dipped in alcohol followed by a brief pass through the flame tospeed the evaporation process.

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Term
Fall
Professor
jane smith
Tags
Growth medium, Agar plate, Petri dish, F Agar, Microbiology Society

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