Replication - Replication (1/25-1/30) Watson and Crick...

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Replication (1/25-1/30) Watson and Crick suggested a “copying mechanism” to DNA. Semi conservative DNA: TTCAGA TTCAGA ½ old and ½ new AAGTCT AAGTCT AAGTCT ½ old and ½ new TTCAGA Conservative DNA: TTCAGA TTCAGA All new AAGTCT AAGTCT AAGTCT All old TTCAGA Prokaryotes (i.e. E.coli ) have no nuclear envelope/nucleus Strands are separated for replication – start at the origin of separation DNAA – protein that attaches to a DNAA box. Boxes bend the DNA (add pressure). This happens at AT regions because they are easier to separate due to 2 H bonds instead of 3 in CG region. Helicase binds at each fork (bubble) to separate the strands of the DNA. Melt = strand separation = unwind = denature Topoisomerase – can break Phosphate in either strand. Turns DNA around to take out the twists. Okazaki fragments are created on the lagging strand during replication with an RNA primer at the 5' end. Small breaks occur between fragments as the bubble gets bigger which need to be
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Replication - Replication (1/25-1/30) Watson and Crick...

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