2_22 - lecture 2/22/07 Bacterial Genetics Why study...

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lecture 2/22/07 Bacterial Genetics Why study bacteria? They’re simple, they’re pathogens (the more we understand, the more we can fight them!), used to clone, produce proteins or pharmaceuticals for industrial use, etc. Culturing Bacteria Broth- water, carbon source (usually glucose), and salts (all other atoms needed by cells to make what they make; aren’t found in glucose, water, or air). This is used because it is very easy to grow bacteria in a liquid. Because every cell is an individual in that broth, there is no way to id different characteristics of the bacteria. In a broth, you can’t select what bacteria you want, and which you don’t want. So it is not so useful for selection. So you want to use… Agar- water, carbon source (probably glucose), salts, and agar (kind of like gelatin and add it to a powder as a hot liquid: while it’s still hot, you can pour it into a form and as it cools, it turns into a semisolid; then take a small amount of bacteria and spread it on the surface). This allows the bacteria to land as individuals on that broth. This is especially good because they can’t move! Then, as they grow and divide and divide, you can see clumps of them-
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2_22 - lecture 2/22/07 Bacterial Genetics Why study...

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