Genetics_Notes - Genetics 19/01/2007 15:35:00 DNA Structure...

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Unformatted text preview: Genetics 19/01/2007 15:35:00 DNA Structure The substance of heredity 1928 Griffiths Experiment 1 Mice infected with Streptococcus pneumonia A. Smooth had polysaccharide coating which protected virus B. Rough had no coating and was venerable Living smooth mouse died isolated smooth virus in culture Living rough mouse survived no virus isolated in a culture Dead smooth mouse survived no virus in culture Living rough & dead smooth Mouse died isolated smooth virus in culture Bacteria reproduces asexually Dead S cells cannot reproduce Rough cells changed into smooth cells Rough transformed b/c smooth cells Transforming Principle Transforming Principle Scientist believed the transforming principle got into S cells Griffith saw genetic material and gene link in the culture grown They influence traits Inheritable 1940s -- Avery, MacLeod, & McCarty Experiment 2 -- Isolate & characterize the Transforming Principle They believed they could find chemical responsible for transforming By doing this they could identify it They chopped up Dead Smooth Cells and fractionated it into different parts Polysaccharide Fraction Lipid Fraction RNA Fraction Lipid Fraction DNA Fraction Protein Fraction- S Polysaccharide Fraction mixed w/ live R no S grew in culture Polysaccharides do no have Trans Princ - S Lipid Fraction mixed w/ live R no S colonies grew in culture Lipids do not contain the Trans Princ - S Protein Fraction mixed w/ live R no S grew in culture Protein Fractions do not contain the Trans Princ - S RNA Fraction mixed w/ live R no S colonies grew RNA does not contain Trans Princ - S DNA Fraction mixed w/ live R Smooth colonies grew in culture DNA Fraction contains Trans Princ TP = DNA Chance of Contamination Maybe TP is a contaminate in the DNA Fraction Took DNA Fraction and examined it closely Rough No smooth R + Smooth Smooth R + DNA fraction + DNAse (enzyme - breaks down DNA) No Smooth DNAse must have destroyed the TP Transforming principle is made out of DNA R + DNA fraction + RNAse Smooth Cells Grew R + DNA fraction + Proteases Smooth Cells Grew 1950s Hershey & Chase (DNA) Deoxyribonucleic acid and (RNA) Ribonucleic acid DNA Polymers Chains of units (nucleotides) Nucleotides strands of units which are covalently bonded o Nitrogen Base + Sugar + 1-3 phosphate groups DNA vs. RNA Difference in DNA and RNA is the attached Sugar 5 sided ring, 5 carbon sugar & 1 point is oxygen Corner is unlabeled because it is an understood carbon Carbon should be bonded to four things Deoxyribose is a de oxygenated ribose Bases - Purines (6sided & 5 sided)...
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This note was uploaded on 04/15/2008 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

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Genetics_Notes - Genetics 19/01/2007 15:35:00 DNA Structure...

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