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Unformatted text preview: unbound antibodies. Run the double labeled mixed cell suspension through the FACS instrument. The instrument will separate the cell populations based on their fluorescence (e.g., will separate red cells from green cells). 3. What is the end product or result? If the separation works, you would then have 2 separate populations of live, healthy cells that could be used for in vitro experiments. 4. How can the effectiveness of the FACS based separation be verified? By RT-PCR and by direct observation with immunofluorescence microscopy (Mukouyama does this in fig 6D and in fig 7ABCD.). So, what do you think? Did Mukouyama successfully separate neurons from Schwann cells by the FACS technique? What is the experimental evidence that supports the answer you have chosen?...
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This note was uploaded on 01/24/2009 for the course BIS 104 taught by Professor Scholey during the Fall '08 term at UC Davis.
- Fall '08
- cell biology