lect_9Nervecellcommunication1_2slides06W

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1 Nerve Cells and Intercellular Communication
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2 Signal is a fast change in membrane potential Two neurons from the brain shows that they receive many synapses. Fluorescent tagged antibody to MAP2 Fluorescent tagged antibody to synaptotagmin
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3 EM of a synapse in the brain
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4 Neuronal Circuits This is the knee jerk reflex and is monosynaptic Development of Electrical Energy Fast changes in V m used for signaling
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5 How to measure membrane potentials Normally the ions K and Cl are close to equilibrium, but Na is way out of equilibrium! Ionic channels
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6 Relative ion permeabilities Conduction along axons
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7 The action potential is generated by change in relative numbers of open channels Depolarization
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Unformatted text preview: hyperpolarization Voltage controls the opening and closing of ion channels. 8 Conduction of the action potential Inactivation assures conduction in one direction only Evolution has insured fast conduction 9 Single ion channels behavior Patch-clamping 10 Types of Patch clamping Single channel kinetics 11 Molecular Biology of Ion Channels 1. Isolate the channel protein 2. Get N-term sequence usually 3. Make redundant nucleotide sequence 4. Isolate clones and mRNA 5. Inject mRNA into frog oocytes 6. Do site -specific mutagenesis 7. Test affects in oocytes Frog Oocyte 12 Shaker mutation Transmembrane structures...
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