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Unformatted text preview: 1 NAME (LAST, FIRST, ID#)____________________________________ TA (SECTION)____________________________________ MCDB 100 VALENTINE MIDTERM (270 pts) Tues 2/14/2006 Instructions: Please read carefully! 1. Write your answers in pen only on the space provided i.e. on the lines, not between them, under the questions. No credit will be given for material in any other space. Honest! 2. Write legibly. We can't give points if we can't read your answers. 3. Show all calculations. 1. (40 pts) define the following: (i) Hydropathy plot: graph of relative hydrophobicity of aa's to predict _____ possible transmembrane protein regions _____________________________ (ii) Gibb’s Free energy: quantity associated with a substance ability to do useful work ____________________________________________________ (iii) J MAX : Facilitated transport flux at infinite substrate concentration ____ ______________________________________________________________ (iv) PIP 2 : intracellular precursor signal molecule electrical energy per _____ charge across the cell membrane ___________________________________ (v) Cell line: cells grown in tissue culture undergo a crisis from which only a few cells survive and then become immortal ________________________ (vi) Cristae: mitochondrial inner cell membrane containing F F 1 ATPase __ ________________________________________________________________ (vii) Dielectric constant: it is a measure of a substance’s ability to shield charges or to be polarized. _________________________________________ (viii) Active transport: movement up a chemical gradient requiring free ____ energy __________________________________________________________ 2 NAME (LAST, FIRST)_______________________________ TA (SECTION)____________________________________ 1. (Cont.) (ix) Proton motive force: measure of the free energy available to synthesize mitochondrial ATP ________________________________________________ (x) HGRPT: enzyme needed to utilize hypothxanthine for the salvage ______ pathway of nucleotide biosynthesis __________________________________ 2. (35 pts) How can FRET be used to determine if the catalytic subunit of PKA, but not its regulatory subunit, is transferred into the nucleus?...
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This note was uploaded on 01/24/2009 for the course MCDB 100 taught by Professor Olague during the Winter '07 term at UCLA.
- Winter '07
- cell biology