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Unformatted text preview: were calculated. 3. What are the major findings (i.e., results)? The figures in Table I account for the denaturing action of 8 M urea. The tyrosine side chain seems to be slightly more hydrophobic than the phenylalanine side chain. 40% ethanol and 7 M urea produce about an equal extent of unfolding. The 8 M urea is to some extent more complete. 4. What are the major conclusion(s)? Aqueous urea solutions accommodate non-polar side chains of amino acids more readily than water does. The constituent parts of the amino acid molecule interact with the solvent independently. Urea solutions are better at solubilizing aromatic side chains, such as phenylalanyl and tyrosyl groups. 5. What points about the paper are confusing or unclear? I do not really understand the experiment behind the process of measuring the solubility of different amino acids. Also, the calculation behind finding the free energy is confusing....
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This note was uploaded on 01/25/2009 for the course BIOL 501 taught by Professor Garrett during the Spring '09 term at UVA.
- Spring '09