{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

HW #3 biochem - Jin Bae Homework#3 1 After the inhalation...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Jin Bae Homework #3 1. After the inhalation of Bacillus anthracis, which leads to infection, it is very important to identify the inhibitors of anthrax lethal factor to treat it with antibiotics in its early stage. The purpose of this experiment was to identify the small molecule inhibitors of anthrax lethal factor by running numerous experiments. 2. First experiment: High-throughput screening and hit validation by using HPLC- based assay. The purpose of this experiment was to screen and identify compounds that inhibit LF activity. The experiment was carried out in this manner. After the LF cleaves an optimized peptide, the fluorescence increased. After the standardization of the high-throughput assay, the 1,990 compounds in the NCL diversity set were tested; then more than 75% inhibition were selected. Finally, by using an HPLC-based assay, more than 50% inhibitions were selected. Second experiment: This experiment involved identifying the structure of anthrax LF inhibitors. Identification of 19 compounds with greater than 50% LF that were
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern